functional restriction - ορισμός. Τι είναι το functional restriction
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Τι (ποιος) είναι functional restriction - ορισμός

Restriction maps; Restriction mapping

restriction enzyme         
  • A palindromic recognition site reads the same on the reverse strand as it does on the forward strand when both are read in the same orientation
CLASS OF ENZYMES THAT CLEAVES DNA INTO FRAGMENTS AT OR NEAR SPECIFIC RECOGNITION SITES WITHIN THE MOLECULE KNOWN AS RESTRICTION SITES
Restriction enzymes; Restriction endonuclease; Restriction Enzyme; Restriction nuclease; Restriction endonucleases; Dna restriction-modification enzymes; Dna restriction enzymes; Deoxyribonuclease hindiii; Restriction Enzymes; Restriction Endonucleases; Restriction enzyme, endonuclease; Deoxyribonucleases, type i site-specific; Deoxyribonucleases, type ii site-specific; Deoxyribonucleases, type iii site-specific; Restriction-enzyme cutting site; Site-specific endonuclease; Restriction Endonuclease; Artificial restriction enzyme; Restrictase; Molecular scissors
(also restriction endonuclease)
¦ noun Biochemistry an enzyme with the property of cleaving DNA molecules at or near a specific sequence of bases.
Restriction (mathematics)         
  • The function <math>x^2</math> with domain <math>\mathbb{R}</math> does not have an [[inverse function]]. If we restrict <math>x^2</math> to the non-negative [[real number]]s, then it does have an inverse function, known as the [[square root]] of <math>x.</math>
MATHEMATICAL FUNCTION
Function restriction; Restriction of a function; Restricted function; ⩤; ⩥; Restriction of a map; Extension of a function
In mathematics, the restriction of a function f is a new function, denoted f\vert_A or f {\restriction_A}, obtained by choosing a smaller domain A for the original function f.
Restriction enzyme         
  • A palindromic recognition site reads the same on the reverse strand as it does on the forward strand when both are read in the same orientation
CLASS OF ENZYMES THAT CLEAVES DNA INTO FRAGMENTS AT OR NEAR SPECIFIC RECOGNITION SITES WITHIN THE MOLECULE KNOWN AS RESTRICTION SITES
Restriction enzymes; Restriction endonuclease; Restriction Enzyme; Restriction nuclease; Restriction endonucleases; Dna restriction-modification enzymes; Dna restriction enzymes; Deoxyribonuclease hindiii; Restriction Enzymes; Restriction Endonucleases; Restriction enzyme, endonuclease; Deoxyribonucleases, type i site-specific; Deoxyribonucleases, type ii site-specific; Deoxyribonucleases, type iii site-specific; Restriction-enzyme cutting site; Site-specific endonuclease; Restriction Endonuclease; Artificial restriction enzyme; Restrictase; Molecular scissors
A restriction enzyme, restriction endonuclease, or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class of the broader endonuclease group of enzymes.

Βικιπαίδεια

Restriction map

A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes. In molecular biology, restriction maps are used as a reference to engineer plasmids or other relatively short pieces of DNA, and sometimes for longer genomic DNA. There are other ways of mapping features on DNA for longer length DNA molecules, such as mapping by transduction.

One approach in constructing a restriction map of a DNA molecule is to sequence the whole molecule and to run the sequence through a computer program that will find the recognition sites that are present for every restriction enzyme known.

Before sequencing was automated, it would have been prohibitively expensive to sequence an entire DNA strand. To find the relative positions of restriction sites on a plasmid, a technique involving single and double restriction digests is used. Based on the sizes of the resultant DNA fragments the positions of the sites can be inferred. Restriction mapping is a very useful technique when used for determining the orientation of an insert in a cloning vector, by mapping the position of an off-center restriction site in the insert.